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Soft-rot bacterial pathogen, Erwinia sp., was isolated from Chinese cabbage tissue showing soft-rot symptom. This bacterial strain caused soft-rot to Chinese cabbage and potato, and identified as Erwinia chrysanthemi PY35(Ech PY35). Ech PY35 have extracellular CMCase, pectinase, pectate lyase, and protease activity, but not hemicellulase activity. The results of the microscopy showed that Chinese cabbage tissue and potato tissue were macerated by infection of Ech PY35. In analysis of the CMCases activity in the total protein of Ech PY35, three CMCases were detected as intracellular protein while two CMCases were as extracellular protein by CMC-SDS-PAGE direct stain method.
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